373 words · auto-generated from the episode video
1:01:46Okay, you take your DNA. Now, your DNA is going to have AT um ATGC's all over. Some of the C's, some of the cytoines is going to have that methyl group. Okay. >> Those little red pointies. >> Yeah, those little red pointies here. Here as well. They're little like red tags on the C's, right? There's some C's that are that are like blue. And then there's other C's you can tell that are not. And those are like normal cytosines that aren't methylated, right? What you're going to do is you're going to treat it with a detergent effectively, okay? A chemical treatment that is going to convert unmethylated cytoines into uricils. Use U is the letter that is used in RNA. Okay? So, it's going to leave the the cytoines with the methyl
1:02:27alone, but it's going to change the season to use. Okay? And then I just sequence like normal, >> right? >> Like like I used to, >> right? >> Okay. Right. >> Because I'm good at sequencing. I'm not good at the the like finding where the methyl is. But this is one way, right? Because I've just I've taken all the ones that don't have the methyl groups. I've turned them into another letter. And then now I just sequence like normal. And I'm like, "Oh, this is a U. The DNA shouldn't have a U. That's probably where the the the C was, right? Or I can like I can I can sequence the original gene and then I can sequence the gene after um treating it with this detergent and wherever the difference is, that's where the C's are. Wherever the C remains, that's where the methyl groups are.
1:03:08>> Right. And then now we know where the methylated cytosine is. >> Yeah. Because any cytosine that survived had to have had a methyl group on it. >> That makes sense. which now gives us a different map [clears throat] >> to to analyze that's not just in indistinguishable C's >> methylated versus non-methylated. >> Now you can imagine treating a molecule like DNA with chemical is not a good idea. Okay? And of course not because what ends up happening is something