Allelic phasing: which parent did it come from?

1:05:02the epigenetic mark came from. That's that's why the the maze thing was was such a big deal, right? Because I could tell like what was happening, right? >> But in order to do that, what that requires is each of these short reads to have a mutation from the par from one parent and not from the other. Right? It's like it's like, oh, this part is a T. I know that the mom had the T here and not the dad had a C here. So I know that this part comes from the mom. But if I have short enough reads that are 150, the probability of getting one of these single nucleotide permutations in my short read is very low. >> Mhm. >> Right. >> Because the shorter the thing is, the probability of something going wrong is >> right >> is not that high. >> So the methylation could be different

1:05:44between two identical, but I don't know which one came from the mom, which one came from the dad. >> That's actually a really good point. And that's that's fundamentally >> Yeah. And fundamentally, the whole point of what you're trying to do is you need to be able to make that that connection. >> Yeah. I I'd like to know which paternal chromosome it came from from the entire whole. >> It's like you have two white puzzles. One is the Beatles white album and one is whatever the White Stripes white album >> and it's like the white piece >> and now you're taking the white piece from what? >> Yeah. It's like they're all white and but is this the white album? Like and so now you're So >> that's exactly right. That's that's a good good analogy. Exactly. Okay. So that's been the problem. I don't know how to do it. It's like we've been relying on the sequencing technology that gives us the sequence of DNA, but

1:06:27it's not designed for methylation reads, >> right? Which which is key for us to be able to identify, >> you know, where we're going to see these mutations that cause the dominant to get suppressed. >> Yeah. Yeah. And all of this weird shenanigan >> all the weird stuff we just all the weird stuff >> it kind of deres at least in part from from this >> from understanding where which chromosome is the parent right [clears throat] which chromatid came from which parent okay so now we get to why it's happening now the Oxford nanopore revolution okay this is an